Proteomics. Tissue-based map of the human proteome.
Level 5 - mechanism / opinion, no new human data
Bench research / molecular profiling study
PubMed 25613900 · doi:10.1126/science.1260419
What was done
The authors generated a spatial and quantitative map of the human tissue proteome across 32 different tissues and organs by integrating quantitative transcriptomics with tissue microarray-based immunohistochemistry down to the single-cell level. They profiled the human secretome, membrane proteome, druggable proteome, cancer proteome, and metabolic functions, integrating findings into an open web database.
What was found
The tissue-based analysis detected expression for more than 90% of putative protein-coding genes across the 32 examined tissues and organs. Specific quantitative expression metrics and numerical breakdowns for individual protein classes were not reported in the abstract.
Why it matters
This study provides a comprehensive reference atlas of spatial protein localization and gene expression across major human tissues, facilitating basic biological discovery and drug target evaluation.
Limits
The abstract provides minimal quantitative details beyond the broad >90% detection figure. The number of individual human donors, demographic details, donor health conditions, and potential antibody cross-reactivity or validation constraints are not described in the abstract.
Cited by
- supports The human genome contains about 20,000 genes, of which about 15,000 are turned on.