Henderson · Neuropharmacology 2015 · narrative review · n=?

Inside-out neuropharmacology of nicotinic drugs.

Cited 102 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review of mechanistic and cellular pharmacology without primary clinical data

PubMed 25660637 · doi:10.1016/j.neuropharm.2015.01.022 · record verified 2026-08-29

What was done

This narrative review summarizes current mechanistic evidence on how nicotine and other nicotinic agonists act inside intracellular organelles to alter neuronal nicotinic acetylcholine receptor (AChR) processing, a concept termed "inside-out pharmacology."

What was found

The abstract reports no numerical data or effect sizes. It describes a mechanistic model wherein cell-permeable nicotinic ligands bind immature intracellular AChR oligomers, induce conformational changes, strengthen subunit interactions, promote maturation into stable pentamers, increase secretory pathway trafficking, and modulate endoplasmic reticulum stress and the unfolded protein response, ultimately resulting in receptor upregulation at the plasma membrane.

Why it matters

This framework distinguishes intracellular pharmacological chaperoning from classical cell-surface receptor activation, providing a mechanistic explanation for chronic nicotine-induced receptor upregulation.

Limits

The review provides theoretical and mechanistic synthesis rather than systematic review or meta-analysis. The abstract provides no quantitative data, and findings are based on cellular and bench models rather than human clinical outcomes.

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