Orihuela · British journal of pharmacology 2016 · narrative review with in vitro laboratory experiments · n=?

Microglial M1/M2 polarization and metabolic states.

Cited 2102 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review with in vitro cell culture data (mechanism-based / bench research)

PubMed 25800044 · doi:10.1111/bph.13139 · record verified 2026-08-26

What was done

The authors reviewed published literature on microglial M1 and M2 phenotype polarization and its relationship to mitochondrial energy metabolism. They also presented supplementary in vitro laboratory data examining mitochondrial metabolic shifts in primary microglia and the BV-2 microglial cell line following stimulation with LPS (M1) or IL-4/IL-13 (M2).

What was found

The review describes that M1 polarization (stimulated by LPS or IFN-γ) promotes pro-inflammatory cytokine expression and is associated with a metabolic shift from oxidative phosphorylation to aerobic glycolysis, while M2 polarization (stimulated by IL-4/IL-13) supports inflammation resolution and tissue repair. The abstract reports no numerical data, effect sizes, or test statistics.

Why it matters

Understanding the metabolic reprogramming underlying microglial activation states clarifies mechanistic links between neuroinflammation, mitochondrial function, and neurodegenerative disease processes.

Limits

The abstract provides no quantitative measurements, sample sizes, or statistical metrics. The findings rely on narrative synthesis and in vitro cell culture systems (primary microglia and BV-2 cells), which cannot fully replicate human in vivo central nervous system biology.

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