Layman · The American journal of clinical nutrition 2015 · Narrative review · n=?

Defining meal requirements for protein to optimize metabolic roles of amino acids.

Cited 136 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review and expert perspective synthesizing literature without original trial data or systematic search methodology.

PubMed 25926513 · doi:10.3945/ajcn.114.084053 · record verified 2026-08-29

What was done

This is a narrative review synthesizing literature on essential amino acids as meal-based metabolic signals (including leucine-induced mTORC1 activation, GCN2, GLP-1, PYY, serotonin, and insulin). It examines the concept of a per-meal protein threshold for skeletal muscle protein synthesis and evaluates how advancing age, physical inactivity, and resistance exercise alter these requirements.

What was found

The abstract presents synthesized benchmarks from prior literature rather than new experimental data. It reports that older adults require daily protein intakes of ≥1.0 g/kg (compared to standard recommendations of 0.8 g/kg) and identifies anabolic and metabolic benefits from consuming at least 20–30 g of protein at a given meal. Resistance exercise is described as increasing the efficiency of essential amino acid use for muscle anabolism and lowering the per-meal threshold needed to stimulate protein synthesis.

Why it matters

It emphasizes shifting dietary guidance from single daily protein totals to per-meal protein redistribution (e.g., 20–30 g across three meals) to overcome age- or inactivity-related declines in muscle protein synthesis.

Limits

The abstract describes a narrative review without original data, systematic review methodology, or meta-analytic pooling. Specific effect sizes, confidence intervals, sample sizes, and participant characteristics of the underlying studies are not reported in the abstract.

Cited by