Polyzos · Human reproduction (Oxford, England) 2015 · retrospective cross-sectional study · n=4894

Thyroid autoimmunity, hypothyroidism and ovarian reserve: a cross-sectional study of 5000 women based on age-specific AMH values.

Cited 92 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional analysis of retrospective clinical cohort data

PubMed 25948573 · doi:10.1093/humrep/dev089 · record verified 2026-08-26

What was done

A retrospective cross-sectional study evaluated 4,894 consecutive women (from 5,076 screened) at a fertility center who had same-day serum measurements of anti-Müllerian hormone (AMH), free thyroxine (FT4), thyroid-stimulating hormone (TSH), and anti-thyroperoxidase antibodies (anti-TPO). Women were categorized by age-specific AMH values into normal reserve (n = 3,929), low reserve (n = 487), or high reserve (n = 478), and thyroid parameters were compared across groups.

What was found

FT4 and TSH levels were comparable across reserve categories (P = 0.611 and P = 0.811, respectively). Anti-TPO positivity showed no significant difference among low (12.1%), normal (10.3%), and high (9.8%) ovarian reserve groups (P = 0.423). The prevalence of overt or subclinical hypothyroidism was also similar across groups: 4.1% in low, 4.6% in normal, and 3.8% in high reserve (P = 0.645). In subgroup analysis of low reserve causes, women with genetic etiology had higher rates of overt (25.0% vs. 3.2%, P = 0.002) and subclinical hypothyroidism (18.8% vs. 1.6%, P = 0.004) compared to those with unexplained low reserve.

Why it matters

These findings suggest that routine thyroid autoimmunity and mild thyroid dysfunction do not explain low age-specific ovarian reserve in the general fertility population.

Limits

The retrospective cross-sectional design cannot establish causality or track longitudinal AMH changes over time. Findings from a single fertility center may not generalize to the broader population, and only anti-TPO antibodies were measured rather than broader autoimmune markers.

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