· Nature 2015 · cross-sectional genomic sequencing and variant discovery study · n=2,504

A global reference for human genetic variation.

Cited 20319 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional descriptive genomic reference study (level 4 by design analogy, non-clinical).

PubMed 26432245 · doi:10.1038/nature15393 · record verified 2026-08-27

What was done

Low-coverage whole-genome sequencing, deep exome sequencing, and dense microarray genotyping were conducted in 2,504 individuals representing 26 distinct global populations to reconstruct genomes and phase genetic variants into haplotypes.

What was found

The project characterized over 88 million variants, comprising 84.7 million single nucleotide polymorphisms (SNPs), 3.6 million short insertions/deletions (indels), and 60,000 structural variants. This resource captured >99% of SNP variants with a frequency >1% across diverse ancestries.

Why it matters

It establishes a foundational, high-density reference map of common human genetic variation and phased haplotypes to support genome-wide association studies and human population genetics.

Limits

Whole-genome sequencing was conducted at low coverage, limiting the sensitivity for detecting ultra-rare variants and resolving complex structural variations. Clinical outcomes and phenotypes were not evaluated.

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