Donkin · Cell metabolism 2016 · Comparative cross-sectional and before-and-after cohort study · n=?

Obesity and Bariatric Surgery Drive Epigenetic Variation of Spermatozoa in Humans.

Cited 532 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional comparison and uncontrolled prospective before-and-after cohort study

PubMed 26669700 · doi:10.1016/j.cmet.2015.11.004 · record verified 2026-08-29

What was done

Epigenomic profiling (histone positioning, small non-coding RNA expression, and DNA methylation) was performed on spermatozoa from lean and obese men. In a separate cohort of men with morbid obesity, changes in sperm DNA methylation patterns were evaluated before and after bariatric surgery-induced weight loss.

What was found

Histone positioning was similar between lean and obese men, but small non-coding RNA expression and DNA methylation patterns differed markedly. Following bariatric surgery, significant remodeling of sperm DNA methylation was observed, notably at genomic regions implicated in the central control of appetite. The abstract reports no numerical values, effect sizes, or p-values.

Why it matters

This study provides evidence that human sperm epigenetic marks are dynamically altered by weight loss, pointing to a potential mechanism for nongenetic paternal transmission of metabolic traits.

Limits

Sample sizes, participant characteristics, and specific effect sizes are not reported in the abstract. The study is observational without a non-surgical weight-loss control group mentioned, and functional effects on offspring phenotype or clinical outcomes were not assessed.

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