Age-Specific Normal Reference Range for Serum Anti-Müllerian Hormone in Healthy Chinese Han Women: A nationwide Population-Based Study.
Level 4 - case-series / case-control
Cross-sectional normative reference range study
PubMed 26763552 · doi:10.1177/1933719115625843
What was done
A multicenter cross-sectional study recruited 2,055 women aged 20 to 55 years via advertisements across six regions in China, of whom 1,590 met inclusion criteria for the reference population. Baseline serum anti-Müllerian hormone (AMH) was measured using the Beckman Coulter Gen II assay. Antral follicle counts and follicular-phase levels of FSH, LH, estradiol, testosterone, prolactin, and progesterone were also evaluated.
What was found
Serum AMH declined with age according to the quadratic model log(AMH) = -1.970 + 0.296 × Age - 0.006 × Age². Median AMH values and 5th to 95th percentiles were: 6.23 ng/mL (2.06 to 12.66 ng/mL) for age 20 to <25; 5.65 ng/mL (1.77 to 13.83 ng/mL) for age 25 to <30; 4.55 ng/mL (1.48 to 11.45 ng/mL) for age 30 to <33; 3.74 ng/mL (0.87 to 9.76 ng/mL) for age 33 to <37; 2.78 ng/mL (0.56 to 9.49 ng/mL) for age 37 to <40; and 1.09 ng/mL (0.08 to 5.70 ng/mL) for age 40 to <55. AMH was positively correlated with antral follicle count, testosterone, LH, prolactin, and progesterone, and negatively correlated with BMI and FSH; estradiol showed no significant correlation. Controlling for age and BMI rendered the correlation with prolactin non-significant.
Why it matters
This study establishes age-specific reference ranges and nomograms for serum AMH in healthy Chinese Han women using the Beckman Coulter Gen II assay.
Limits
The cross-sectional design cannot track individual longitudinal trajectories of AMH decline. Recruitment via advertisement introduces potential selection bias. Findings are specific to the Beckman Coulter Gen II assay and Chinese Han population.
Cited by
- supports A normal AMH level for women in their 20s and 30s is up to 6 ng/mL, whereas for women in their 40s it is typically less than 1 ng/mL.