Choi · Cell reports 2016 · Preclinical animal study and preliminary randomized controlled trial · n=?

A Diet Mimicking Fasting Promotes Regeneration and Reduces Autoimmunity and Multiple Sclerosis Symptoms.

Cited 514 times in the scientific literature.

Level 2 - randomized trial

Includes preliminary human data from a registered randomized controlled trial alongside preclinical animal models

PubMed 27239035 · doi:10.1016/j.celrep.2016.05.009 · record verified 2026-08-30

What was done

Researchers evaluated periodic 3-day cycles of a fasting-mimicking diet (FMD) in mouse models of multiple sclerosis, specifically experimental autoimmune encephalomyelitis (EAE) and cuprizone-induced demyelination. They measured clinical disease severity, immune cell subsets (Tregs, TH1, TH17, antigen-presenting cells), corticosterone levels, and oligodendrocyte precursor cell regeneration/remyelination. Additionally, they gathered preliminary safety, feasibility, and efficacy data for an FMD or a chronic ketogenic diet in patients with relapsing-remitting multiple sclerosis (RRMS; NCT01538355).

What was found

In the EAE mouse model, FMD cycles reduced clinical disease severity in all treated mice and completely reversed symptoms in 20% of animals. Mechanistically, FMD increased corticosterone and regulatory T (Treg) cell numbers while reducing pro-inflammatory cytokines, TH1 cells, TH17 cells, and antigen-presenting cells. FMD also stimulated oligodendrocyte precursor cell regeneration and axonal remyelination in both EAE and cuprizone models. In human RRMS patients, preliminary trial data showed that FMD and a chronic ketogenic diet were safe, feasible, and potentially effective, though specific numerical outcomes for the human cohort were not reported in the abstract.

Why it matters

This study provides evidence that periodic fasting-mimicking diets can dual-target autoimmune pathology and remyelination pathways in preclinical neuroinflammation, establishing early feasibility for dietary metabolic interventions in multiple sclerosis.

Limits

The human trial findings are described as preliminary without reporting sample size, baseline characteristics, dropout rates, or numerical effect sizes in the abstract. Most efficacy and mechanistic findings rely on mouse models (EAE and cuprizone), which do not fully replicate human multiple sclerosis pathology.

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