Pietrocola · Cancer cell 2016 · preclinical animal experiment · n=?

Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance.

Cited 545 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal (mouse) research without human subjects

PubMed 27411589 · doi:10.1016/j.ccell.2016.05.016 · record verified 2026-08-30

What was done

Evaluated the effects of short-term fasting and caloric restriction mimetics (CRMs)—specifically the ATP citrate lyase inhibitor hydroxycitrate and spermidine—in mice bearing mutant KRAS-induced lung cancers and other tumors. Researchers assessed tumor mass, chemotherapy-induced tumor growth inhibition, regulatory T-cell levels in the tumor bed, and the necessity of tumor autophagy competency and T lymphocytes.

What was found

The abstract provides directional findings without quantitative data or effect sizes. Hydroxycitrate depleted regulatory T cells from autophagy-competent, but not autophagy-deficient, mutant KRAS-driven lung tumors in mice, reducing tumor mass and improving immunosurveillance. Short-term fasting and chemically unrelated CRMs (hydroxycitrate, spermidine) enhanced chemotherapy-induced tumor growth inhibition in vivo. This effect required both host T lymphocytes and tumor autophagy competency, and was accompanied by tumor-bed regulatory T-cell depletion.

Why it matters

Identifies a potential immunological mechanism linking nutrient deprivation, caloric restriction mimetics, and tumor autophagy to enhanced chemotherapy efficacy via regulatory T-cell reduction.

Limits

The findings are derived entirely from preclinical mouse models; translational efficacy, optimal dosing, and safety in humans are unestablished. The abstract reports no quantitative values, sample sizes, or statistical confidence intervals.

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