Elango · Advances in nutrition (Bethesda, Md.) 2016 · narrative review · n=?

Protein and Amino Acid Requirements during Pregnancy.

Cited 160 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review summarizing previous tracer studies and animal models

PubMed 27422521 · doi:10.3945/an.115.011817 · record verified 2026-08-26

What was done

This review evaluated dietary protein and amino acid requirement determinations across pregnancy, comparing standard factorial estimates with metabolic data obtained using the minimally invasive indicator amino acid oxidation (IAAO) method in women during early (~16 weeks) and late (~36 weeks) gestation, alongside indispensable amino acid requirement data from swine models.

What was found

Current Estimated Average Requirement and RDA recommendations set uniform values of 0.88 and 1.1 g · kg(-1) · d(-1) across all stages of pregnancy. In contrast, IAAO measurements identified average requirements of 1.2 g · kg(-1) · d(-1) in early pregnancy (~16 wk) and 1.52 g · kg(-1) · d(-1) in late pregnancy (~36 wk), representing ~14-18% of total energy intake. Swine models showed that requirements for several indispensable amino acids increase dramatically in late compared to early gestation.

Why it matters

Current single-value dietary recommendations across all gestational stages may underestimate protein needs during the third trimester when maternal and fetal tissue growth peaks.

Limits

The abstract describes a narrative review and does not report sample sizes, confidence intervals, or study quality metrics for the referenced IAAO studies. Evidence for specific indispensable amino acid requirements is derived from swine models rather than direct human trials.

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