Karlikova · Anticancer research 2016 · case-control study · n=253

Circulating Growth and Angiogenic Factors and Lymph Node Status in Early-stage Breast Cancer - A Pilot Study.

Cited 10 times in the scientific literature.

Level 4 - case-series / case-control

Case-control biomarker study comparing cancer patients to healthy controls

PubMed 27466533 · record verified 2026-08-29

What was done

Serum IGF1 and IGFBP3, alongside plasma EGF, VEGF, OPG, and OPN, were measured in 211 patients with malignant breast cancer and 42 age-matched healthy controls. Biomarker levels were compared between cancer patients and controls, between patients with positive versus negative lymph node status, and between patients with and without axillary lymph node dissection (ALND).

What was found

IGF1 and VEGF levels were elevated in patients with lymph node metastases compared to controls (p=0.0179 and p=0.0091, respectively) and in patients who underwent ALND compared to controls (p=0.0337 and p=0.0438, respectively). The abstract does not provide absolute concentration values, effect sizes, diagnostic accuracy metrics, or results for IGFBP3, EGF, OPG, and OPN.

Why it matters

Identifying non-invasive circulating biomarkers could potentially assist in assessing lymph node involvement and reducing unnecessary axillary lymph node dissections in early-stage breast cancer.

Limits

The abstract provides only p-values with no numerical concentrations, confidence intervals, or diagnostic performance measures. Direct statistical comparisons between node-positive and node-negative cancer patients are not reported in the abstract despite being listed in the study methods. As a pilot study, findings require validation in larger prospective cohorts.

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