Victor · Circulation research 1989 · within-subject experimental crossover trial · n=20

Effects of partial neuromuscular blockade on sympathetic nerve responses to static exercise in humans.

Cited 242 times in the scientific literature.

Level 3 - non-randomized controlled study

Non-randomized physiological laboratory crossover study in humans

PubMed 2752552 · doi:10.1161/01.res.65.2.468 · record verified 2026-08-29

What was done

In nine healthy conscious subjects, investigators measured arterial pressure, heart rate, and muscle sympathetic nerve activity via peroneal nerve microelectrodes in a resting leg. Measurements were taken during static handgrip (15% and 30% maximal voluntary contraction) and during attempted handgrip under partial neuromuscular blockade induced by intravenous tubocurarine chloride (0.075 mg/kg). In 11 additional subjects, heart rate responses during curare administration were evaluated following atropine or propranolol administration.

What was found

Attempted handgrip with near-maximal effort under partial neuromuscular blockade generated minimal force. Central command alone significantly increased muscle sympathetic nerve activity, arterial pressure, and heart rate (p < 0.05). The increases in muscle sympathetic nerve activity (+56 +/- 16% over control) and mean arterial pressure (+12 +/- 2 mm Hg) during attempted handgrip were significantly smaller (p < 0.05) than during actual static handgrip at 30% maximal voluntary contraction (+217 +/- 37% and +25 +/- 3 mm Hg, respectively). Heart rate increase during attempted contraction (+18 +/- 2 beats/min) was comparable to actual contraction (+16 +/- 4 beats/min). In the 11 additional subjects, the heart rate response during curare was significantly attenuated by atropine (p < 0.05) but not by propranolol.

Why it matters

This study provides direct microneurographic evidence in humans that central command alone can activate sympathetic outflow and raise blood pressure, though peripheral feedback is required to achieve the full pressor and sympathetic response.

Limits

The sample size is small (n = 9 for microneurography, n = 11 for heart rate pharmacology). Participant demographics, randomization of the exercise order, and baseline absolute values are not reported in the abstract.

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