Moro · Journal of translational medicine 2016 · Randomized controlled trial · n=34

Effects of eight weeks of time-restricted feeding (16/8) on basal metabolism, maximal strength, body composition, inflammation, and cardiovascular risk factors in resistance-trained males.

Cited 734 times in the scientific literature.

Level 2 - randomized trial

Individual randomized controlled trial

PubMed 27737674 · doi:10.1186/s12967-016-1044-0 · record verified 2026-08-29

What was done

Thirty-four resistance-trained males were randomized to an 8-week 16/8 time-restricted feeding protocol (TRF; meals at 1 p.m., 4 p.m., and 8 p.m.) or a normal diet (ND; meals at 8 a.m., 1 p.m., and 8 p.m.) while following a standardized resistance training program. Diets were matched for energy intake (TRF: 2,826 ± 412.3 kcal/day; ND: 3,007 ± 444.7 kcal/day) and macronutrients (~53–55% carbohydrate, ~24–25% fat, ~21–22% protein). Measures included body composition via DXA, thigh and arm muscle area, maximal strength (bench press and leg press), resting energy expenditure, respiratory ratio, and endocrine, inflammatory, and lipid biomarkers.

What was found

Fat mass decreased significantly in TRF compared to ND (p = 0.0448), while fat-free mass, muscle area, and maximal strength were maintained across both groups. TRF resulted in significant decreases in testosterone (p = 0.0476), IGF-1 (p = 0.0397), total leptin (p = 0.0001; not significant after fat mass adjustment), and triiodothyronine (T3), alongside an increase in adiponectin (p < 0.0001) and a decrease in respiratory ratio. No significant changes occurred in resting energy expenditure, TSH, total cholesterol, HDL, LDL, or triglycerides. Absolute numerical values for outcome changes were not reported in the abstract.

Why it matters

This study demonstrates that a 16/8 time-restricted feeding pattern can promote fat loss while preserving muscle mass and strength during resistance training, though accompanied by decreases in anabolic and thyroid hormones.

Limits

The study is limited by a small sample size (n = 34) and an exclusively male, resistance-trained cohort, limiting generalizability to females and untrained populations. The 8-week trial duration cannot determine the long-term impact of hormonal declines (testosterone, IGF-1, T3) on muscular adaptation, and specific numeric effect sizes were omitted from the abstract.

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