Adamovich · Cell metabolism 2017 · Preclinical animal and in vitro laboratory experiment · n=?

Rhythmic Oxygen Levels Reset Circadian Clocks through HIF1α.

Cited 290 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical cellular and rodent experimental study with no human data.

PubMed 27773695 · doi:10.1016/j.cmet.2016.09.014 · record verified 2026-08-29

What was done

The authors monitored tissue oxygen levels continuously in living animals to detect daily fluctuations. They tested whether physiological oxygen cycles synchronized cellular clocks and regulated clock gene expression via HIF1α. Additionally, they assessed whether a short, moderate reduction in oxygen levels altered adaptation speed in wild-type versus HIF1α-deficient mice subjected to a jet-lag protocol.

What was found

Tissue oxygenation in living animals exhibited daily physiological rhythms. In vitro oxygen cycles synchronized cellular circadian clocks and altered clock gene expression in a HIF1α-dependent manner. In vivo, a brief moderate oxygen reduction accelerated adaptation to a new time zone in wild-type mice, but not in HIF1α-deficient mice. The abstract reports no numerical values, effect sizes, oxygen percentages, or sample sizes.

Why it matters

This work identifies oxygen as a physiological resetting cue for mammalian circadian clocks operating through HIF1α. It suggests oxygen-level modulation as a potential non-photic therapeutic strategy for jet lag and circadian disruption.

Limits

The study is entirely preclinical (in vitro and mouse models), limiting direct clinical generalizability to humans. The abstract does not report sample sizes, specific oxygen levels or durations used, or quantitative measures of phase shifting.

Cited by