Kohn · Fertility and sterility 2017 · Retrospective cohort study · n=66

Age and duration of testosterone therapy predict time to return of sperm count after human chorionic gonadotropin therapy.

Cited 67 times in the scientific literature.

Level 4 - case-series / case-control

Retrospective single-arm cohort / case-series without an untreated control group

PubMed 27855957 · doi:10.1016/j.fertnstert.2016.10.004 · record verified 2026-08-27

What was done

A retrospective clinical study evaluated 66 men presenting to an academic male-infertility clinic with infertility following exogenous testosterone (T) use. Treatment consisted of T cessation combined with high-dose human chorionic gonadotropin (hCG) and selective estrogen receptor modulator (SERM) therapy. The primary outcome was achieving a total motile count (TMC) greater than 5 million sperm within 12 months.

What was found

Overall, 46 men (70%) achieved a TMC > 5 million sperm within 12 months. Both increased age and duration of prior T use directly correlated with prolonged time to recovery at 6 and 12 months. Age consistently limited recovery at both time points, whereas duration of T use had less influence at 12 months than at 6 months. Among baseline azoospermic men, 64.8% reached the threshold at 12 months compared with 91.7% of cryptozoospermic men, though baseline count category did not independently predict failure.

Why it matters

This study provides clinical parameters to guide counseling on the timeline of spermatogenesis recovery after testosterone use, highlighting age and treatment duration as key prognostic factors.

Limits

The study is limited by a small sample size (n = 66), a single-center retrospective design, and the absence of an untreated control arm (such as T cessation alone). Pre-testosterone baseline semen parameters and functional fertility outcomes (such as pregnancy rates) were not reported.

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