Desai · Cell 2016 · Preclinical controlled animal study · n=?

A Dietary Fiber-Deprived Gut Microbiota Degrades the Colonic Mucus Barrier and Enhances Pathogen Susceptibility.

Cited 2899 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model (gnotobiotic mice colonized with synthetic human gut microbiota)

PubMed 27863247 · doi:10.1016/j.cell.2016.10.043 · record verified 2026-08-29

What was done

Gnotobiotic mice were colonized with a synthetic human gut microbiota consisting of fully sequenced commensal bacteria. Researchers examined the effects of chronic and intermittent dietary fiber deprivation on the colonic mucus layer and subsequent susceptibility to infection with the enteric pathogen Citrobacter rodentium.

What was found

The abstract reports qualitative mechanistic findings without numerical data. In the absence of dietary fiber, commensal microbes utilized host-secreted mucus glycoproteins as an energy source, degrading the colonic mucus barrier. This degradation permitted increased epithelial access by Citrobacter rodentium, resulting in lethal colitis.

Why it matters

It demonstrates a direct mechanism whereby low-fiber intake causes gut microbes to erode the protective mucus barrier, heightening vulnerability to enteric pathogens.

Limits

The study was conducted entirely in gnotobiotic mice using a simplified synthetic microbiota rather than a natural human community. The abstract provides no specific sample sizes, statistical measures, or quantitative effect estimates. Findings cannot be directly assumed to replicate identically in human intestinal physiology.

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