Thaiss · Nature 2016 · Preclinical animal experiment · n=?

Persistent microbiome alterations modulate the rate of post-dieting weight regain.

Cited 498 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench and animal research (mouse models and fecal transfers).

PubMed 27906159 · doi:10.1038/nature20796 · record verified 2026-08-30

What was done

The authors investigated the role of the intestinal microbiome in post-dieting weight regain using mouse models of recurrent obesity. They performed fecal microbiota transfers from post-diet mice into germ-free mice to test transmissibility of the rapid weight regain phenotype, developed a machine-learning algorithm to predict regain from microbiome features, and tested a flavonoid-based postbiotic intervention to counteract energy expenditure reductions.

What was found

The abstract reports qualitative mechanistic findings without exact numbers or statistical metrics: an altered gut microbiome signature persisted following successful weight loss in mice and drove accelerated weight regain upon re-exposure to obesity-promoting conditions. This phenotype was transmissible to germ-free mice via fecal transplant. The post-diet microbiome was associated with lower flavonoid levels and decreased energy expenditure, and a flavonoid-based intervention ameliorated secondary weight gain.

Why it matters

The study proposes a gut microbiome-mediated 'memory' mechanism contributing to recurrent post-dieting weight regain in mice, offering a potential rationale for microbiome-targeted or metabolite-based therapies.

Limits

The study is entirely preclinical in mice; findings cannot be assumed to translate directly to humans. The abstract does not report sample sizes (n = ?), numerical effect sizes, or confidence intervals.

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