Detection and in vitro metabolism of the confiscated peptides BPC 157 and MGF R23H.
Level 5 - mechanism / opinion, no new human data
In vitro analytical and bench metabolism study with no human subjects
PubMed 28035768 · doi:10.1002/dta.2152
What was done
Researchers characterized two peptides found in confiscated vials (BPC 157 and MGF R23H), evaluated their in vitro metabolic stability in plasma, and validated a weak cation exchange solid phase extraction method to detect BPC 157 and its metabolite in urine.
What was found
In vitro plasma experiments showed MGF R23H is stable and BPC 157 generates a stable metabolite suitable for urinary monitoring. The validated extraction method for urinary BPC 157 achieved a limit of detection of 0.1 ng/mL, precision under 20%, and linearity of r^2 = 0.998. BPC 157 remained stable in urine for at least 4 days.
Why it matters
This assay provides anti-doping laboratories with a validated protocol to detect illicit use of BPC 157 and MGF R23H in urine samples.
Limits
The study tested in vitro plasma metabolism and spiked urine matrices rather than human excretion samples following controlled administration, leaving real-world in vivo pharmacokinetics and detection windows unverified.
Cited by
- supports BPC-157 is included on the World Anti-Doping Agency (WADA) prohibited list.