Hwang · JCI insight 2017 · Single-arm physiological clamp study with 1H-MRS · n=8

The human brain produces fructose from glucose.

Cited 95 times in the scientific literature.

Level 4 - case-series / case-control

Single-arm experimental human physiological study without a separate control group

PubMed 28239653 · doi:10.1172/jci.insight.90508 · record verified 2026-08-27

What was done

Eight healthy adults (4 women, 4 men; mean age 28.8 ± 6.2 years; BMI 23.4 ± 2.6; HbA1C 4.9% ± 0.2%) underwent a 4-hour hyperglycemic clamp protocol targeting a plasma glucose level of 220 mg/dl. Intracerebral glucose and fructose concentrations were monitored throughout the clamp using 1H magnetic resonance spectroscopy (MRS) and analyzed using mixed-effects regression models.

What was found

Intracerebral glucose concentrations rose significantly above baseline from 20 to 230 minutes of the clamp. Intracerebral fructose concentrations also rose significantly above baseline from 30 to 230 minutes. Changes in intracerebral fructose were correlated with intracerebral glucose levels but showed no relationship with circulating plasma fructose levels.

Why it matters

This demonstrates that the human brain can endogenously convert glucose to fructose via the polyol pathway during hyperglycemia, indicating that CNS exposure to fructose does not depend entirely on blood-brain barrier transport of dietary fructose.

Limits

The sample size was very small (n = 8) and restricted to young, healthy, normal-weight individuals. The experimental hyperglycemic clamp (220 mg/dl for 4 hours) represents an artificial condition rather than typical daily glycemic excursions, there was no normoglycemic control cohort, and behavioral or downstream clinical endpoints were not assessed.

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