Medvedeva · Molecular genetics and genomics : MGG 2017 · controlled animal experiment · n=?

Semax, an analog of ACTH (4-7) , regulates expression of immune response genes during ischemic brain injury in rats.

Cited 23 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model (transcriptomic profiling in rats)

PubMed 28255762 · doi:10.1007/s00438-017-1297-1 · record verified 2026-08-27

What was done

Researchers performed genome-wide biochip transcriptome analysis of cerebral cortex tissue from rats subjected to focal cerebral ischemia and treated with either Semax (a synthetic peptide composed of an ACTH(4-7) analog and Pro-Gly-Pro) or Pro-Gly-Pro (PGP) alone. Differentially expressed genes and associated signaling pathways were analyzed using bioinformatics software (Ingenuity iReport).

What was found

The abstract reports no numerical values, fold-changes, or sample sizes. Semax treatment predominantly altered immune response pathways, enhancing antigen presentation signaling, augmenting interferon signaling, and increasing expression of the gene encoding the immunoglobulin heavy chain. It also altered cytokine, stress response, and ribosomal protein-encoding genes. Treatment with PGP alone attenuated immune activity and suppressed central nervous system neurotransmission pathways.

Why it matters

These findings suggest that the neuroprotective action of Semax in ischemic stroke models may be mediated through modulation of neuroimmune signaling pathways rather than purely direct neuronal mechanisms.

Limits

The study was conducted in a rat model of cerebral ischemia, which may not translate directly to human ischemic stroke. The abstract provides no quantitative data, effect sizes, statistical thresholds, dosage, or sample size (n). Functional neurological outcomes and survival were not reported.

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