Roshanzamir · International journal of reproductive biomedicine 2017 · systematic review of animal and human studies · n=27 studies (23 animal, 4 human)

The putative effects of D-Aspartic acid on blood testosterone levels: A systematic review.

Cited 34 times in the scientific literature.

Level 2 - randomized trial

Systematic review including heterogeneous animal and human studies

PubMed 28280794 · record verified 2026-08-29

What was done

Authors conducted a systematic review searching PubMed, ISI Web of Science, ProQuest, and Scopus up to October 2015 for studies evaluating the effect of D-aspartic acid (D-Asp) on blood testosterone levels. Out of 396 retrieved records, 23 animal studies and 4 human studies were included.

What was found

Exogenous D-aspartic acid increased blood testosterone levels in male animal models, dependent on species, sex, and organ site. Results across human studies were inconsistent, and the abstract does not report specific numerical values, effect sizes, or dosages.

Why it matters

Despite its widespread promotion as an over-the-counter testosterone booster for male infertility and athletic enhancement, existing clinical evidence in humans remains sparse and inconclusive.

Limits

Only 4 human studies were identified, and the authors note they were limited in number and of poor quality. The abstract does not provide specific participant characteristics, sample sizes, intervention durations, dosages, or numerical outcomes.

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