Wang · Genome biology 2017 · Preclinical animal controlled study and biomarker development · n=135 mice

Epigenetic aging signatures in mice livers are slowed by dwarfism, calorie restriction and rapamycin treatment.

Cited 331 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research (preclinical mouse model)

PubMed 28351423 · doi:10.1186/s13059-017-1186-2 · record verified 2026-08-30

What was done

Researchers evaluated age-related DNA methylation patterns between mice and humans. Using liver methylomes from 107 mice aged 0.2 to 26.0 months, they constructed an epigenetic aging model for mice. They then tested whether longevity-promoting interventions decelerate this clock by analyzing 28 additional liver methylomes from mice subjected to Prop1 df/df dwarfism, calorie restriction, or dietary rapamycin compared to untreated, wild-type age-matched controls.

What was found

Mice and humans demonstrated moderate conservation of age-altered CpG sites, with both species exhibiting increased methylome disorder during aging. Mice receiving lifespan-extending interventions (dwarfism, calorie restriction, or rapamycin) were significantly younger in epigenetic age than untreated, age-matched controls. The abstract does not report specific numerical values, effect sizes, or p-values.

Why it matters

This study demonstrates that molecular epigenetic clocks operate in mice and can be decelerated by genetic, dietary, and pharmacological lifespan-extending interventions.

Limits

The study is restricted to mouse liver tissue, limiting direct inferences about other tissues or translation to humans. The intervention sample size was small (28 methylomes across three interventions and controls), and specific quantitative metrics of epigenetic age deceleration are not provided in the abstract.

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