Sato · Cell 2017 · controlled animal experiment · n=?

Circadian Reprogramming in the Liver Identifies Metabolic Pathways of Aging.

Cited 369 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research without human clinical data.

PubMed 28802039 · doi:10.1016/j.cell.2017.07.042 · record verified 2026-08-29

What was done

Circadian transcriptomics and biochemical analyses were performed in young and old mice fed either ad libitum or a calorically restricted diet. Investigators evaluated circadian gene expression across the liver, epidermal stem cells, and skeletal muscle stem cells, and measured hepatic NAD+-related metabolites and global protein acetylation oscillations across the daily cycle.

What was found

The abstract reports no numerical values. Caloric restriction reprogrammed the circadian transcriptome in mouse liver in a tissue-specific manner compared to epidermal and muscle stem cells. Genes that gained de novo circadian oscillation under caloric restriction were enriched for SIRT1 targets. Daily rhythmic global protein acetylation, which was lost in old ad libitum-fed mice, was robustly rescued by caloric restriction.

Why it matters

This work demonstrates that dietary restriction can preserve circadian metabolic control during aging by sustaining rhythmic NAD+ metabolism and cyclical protein acetylation in the liver.

Limits

Findings are limited to mice and require confirmation in human metabolic physiology. Exact sample sizes, animal strains, ages, dietary protocols, and quantitative statistical values were not reported in the abstract.

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