Citera · International journal of general medicine 2017 · Diagnostic case-control study · n=2340

Empirical validation of the Horowitz Multiple Systemic Infectious Disease Syndrome Questionnaire for suspected Lyme disease.

Cited 38 times in the scientific literature.

Level 4 - case-series / case-control

Diagnostic case-control and psychometric validation study

PubMed 28919803 · doi:10.2147/IJGM.S140224 · record verified 2026-08-27

What was done

Three studies evaluated the psychometric validity and discriminative ability of the Horowitz Multiple Systemic Infectious Disease Syndrome Questionnaire (HMQ) for suspected Lyme disease. Study 1 assessed construct validity, factor structure, and reliability in 537 patients treated for Lyme disease. Study 2 evaluated convergent and discriminant validity in an online sample of 999 participants (217 self-identified healthy, 782 self-identified active Lyme disease). Study 3 compared 236 patients with confirmed Lyme disease against 568 online healthy controls.

What was found

Factor analysis yielded six latent dimensions, with four corresponding to neuropathy, cognitive dysfunction, musculoskeletal pain, and fatigue. The scale showed acceptable internal consistency via Cronbach's alpha and demonstrated convergent and divergent validity. The HMQ differentiated individuals with Lyme disease from healthy controls, with three migratory pain items (persistent muscular pain, arthritic pain, and nerve pain/paresthesias) identifying confirmed Lyme cases. No numerical performance metrics (such as sensitivity, specificity, or exact reliability coefficients) were reported in the abstract.

Why it matters

The findings provide preliminary validation for the HMQ as an inexpensive screening instrument to help practitioners identify patients who may warrant formal diagnostic testing for Lyme disease.

Limits

The abstract omits numeric diagnostic accuracy metrics (e.g., sensitivity, specificity, AUC). Testing against healthy controls rather than patients with mimicking conditions (e.g., fibromyalgia, chronic fatigue) limits applicability in differential diagnosis. Study 2 relied on unverified, self-reported online diagnoses.

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