Pearson-Leary · Behavioural brain research 2018 · controlled laboratory experiment (in vivo rat and in vitro cell culture) · n=?

Insulin modulates hippocampally-mediated spatial working memory via glucose transporter-4.

Cited 102 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and in vitro laboratory study

PubMed 28943428 · doi:10.1016/j.bbr.2017.09.033 · record verified 2026-08-27

What was done

Male rats were tested on a spontaneous alternation spatial working memory task to evaluate dorsal hippocampal glucose utilization following pharmacological inhibition of GluT4-mediated glucose transport, inhibition of Akt, and intra-hippocampal exogenous insulin administration. Complementary experiments measured insulin-stimulated glucose utilization in neuronal cell cultures with and without GluT4 inhibition.

What was found

The abstract reports no exact numerical values or effect sizes. Glucose utilization increased in the dorsal hippocampus during spontaneous alternation testing. At baseline insulin levels, GluT4 inhibition did not alter task performance, whereas inhibition of Akt impaired performance. When exogenous insulin was administered at a memory-enhancing dose, GluT4 inhibition blocked the cognitive improvement. In neuronal cell culture, insulin increased glucose utilization in a GluT4-dependent manner.

Why it matters

The findings identify GluT4 as a necessary mediator for the cognitive-enhancing effects of elevated hippocampal insulin, distinguishing baseline cognitive processing from insulin-stimulated enhancement.

Limits

The study was conducted entirely in male rats and in vitro cell cultures, limiting direct translation to human physiology. Sample sizes, effect sizes, statistical parameters, and drug dosages were omitted from the abstract, and potential sex-specific differences were not evaluated.

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