Perumalla Venkata · Toxicology reports 2016 · Controlled animal experiment · n=?

Evaluation of the deleterious health effects of consumption of repeatedly heated vegetable oil.

Cited 122 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal study

PubMed 28959587 · doi:10.1016/j.toxrep.2016.08.003 · record verified 2026-08-26

What was done

Peroxide values were measured in repeatedly heated cooking oil (RHCO) compared with unheated or singly heated oil. Wistar rats were administered RHCO (heated repeatedly three times) to evaluate histopathological changes in the jejunum, colon, and liver, alterations in antioxidant enzyme levels, blood biochemistry (glucose, creatinine, cholesterol, protein, and albumin), and hematological parameters.

What was found

RHCO demonstrated a higher peroxide value than unheated or singly heated oil. Histopathology showed significant tissue damage in the jejunum, colon, and liver of rats fed oil heated three times. Treated rats showed altered antioxidant enzyme levels, elevated blood glucose, creatinine, and cholesterol, and decreased total protein and albumin levels. Hematological parameters showed no statistically significant differences between groups. The abstract reports no numerical values, effect sizes, or variances.

Why it matters

Repeated heating of cooking oil is a common culinary practice, and these animal findings illustrate that thermal oxidation products can drive oxidative stress, organ damage, and metabolic disturbances.

Limits

The study was conducted entirely in Wistar rats, limiting direct generalizability to human health. The abstract omits sample size, duration of exposure, specific oil composition, and all quantitative measurements and confidence intervals.

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