Mihaylova · Cell stem cell 2018 · animal laboratory study · n=?

Fasting Activates Fatty Acid Oxidation to Enhance Intestinal Stem Cell Function during Homeostasis and Aging.

Cited 433 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and bench research with no human data

PubMed 29727683 · doi:10.1016/j.stem.2018.04.001 · record verified 2026-08-30

What was done

Evaluated the effects of a 24-hour fast on intestinal stem cell (ISC) function and regeneration in young and aged mice. Investigated the underlying metabolic mechanism by testing pharmacological activation of fatty acid oxidation (FAO) and both acute and long-term genetic disruption of Cpt1a, the rate-limiting enzyme of FAO.

What was found

A 24-hour fast enhanced ISC function in both young and aged mice by upregulating FAO. Pharmacological activation of FAO mimicked the beneficial effects of fasting. Acute Cpt1a disruption blocked fasting-induced enhancements in ISC function, whereas chronic Cpt1a deletion reduced baseline ISC numbers and function. The abstract provides no numerical data or effect sizes.

Why it matters

Identifies fatty acid oxidation as a key metabolic driver through which short-term fasting promotes intestinal stem cell regeneration during aging and homeostasis.

Limits

The study was conducted entirely in mice; human clinical relevance and translational efficacy remain unestablished. The abstract omits sample sizes, specific quantitative endpoints, and statistical metrics.

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