Harding · Current biology : CB 2018 · Preclinical laboratory experiment (neuronal activity tagging and reactivation) · n=?

A Neuronal Hub Binding Sleep Initiation and Body Cooling in Response to a Warm External Stimulus.

Cited 145 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal neurocircuitry study with no human data

PubMed 30017485 · doi:10.1016/j.cub.2018.05.054 · record verified 2026-08-27

What was done

Researchers used c-Fos-dependent neuronal activity tagging to identify and subsequently reactivate populations of neurons in the median preoptic (MnPO) and medial preoptic (MPO) hypothalamic areas activated by external warming to determine their roles in body temperature regulation and sleep induction.

What was found

External warming activated two distinct preoptic neuronal populations. Reactivating GABAergic neurons primarily in the MPO triggered non-rapid eye movement (NREM) sleep without causing a decrease in body temperature. Reactivating nitrergic-glutamatergic neurons across the MnPO and MPO simultaneously induced core body cooling and NREM sleep. The abstract reported qualitative phenotypes without quantitative effect sizes or numerical values.

Why it matters

These findings delineate the specific hypothalamic neural circuits connecting peripheral skin warming to core body cooling and sleep onset, explaining why sleep induction and thermoregulation are mechanistically linked.

Limits

The study is preclinical and conducted in animal models, meaning translational equivalence in humans remains to be confirmed. The abstract does not report sample sizes, specific animal species, quantitative effect sizes, or potential off-target effects of artificial neuronal reactivation.

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