Manning · American journal of human biology : the official journal of the Human Biology Council 2018 · cross-sectional observational study · n=89,926

Sexual dimorphism in the ontogeny of second (2D) and fourth (4D) digit lengths, and digit ratio (2D:4D).

Cited 64 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study by CEBM design analogy

PubMed 30133905 · doi:10.1002/ajhb.23138 · record verified 2026-08-26

What was done

The authors examined sex differences in second (2D) and fourth (4D) digit lengths and digit ratio (2D:4D) across two cohorts: children aged 2 to 18 years (Sample I, n = 680) and adults aged 18 to 30 years (Sample II, n = 89,246). Digit length was determined by direct experimenter measurement in Sample I and direct self-measurement in Sample II. Data were analyzed using two-factor ANOVAs for sex and year-group.

What was found

The abstract reports statistical directions without exact numbers. In both samples, digit length was positively related to age, showing significant main effects of sex and year-group, as well as a significant interaction effect on digit length. Boys had longer digits than girls only after 13 years of age. Men had longer digits than women, with dimorphism increasing from 18 to 30 years. Sex differences in 2D:4D were significant (males < females), but there was no significant effect of age and no interaction effect of age and sex on 2D:4D in either children or adults.

Why it matters

The findings indicate that while individual 2D and 4D digit lengths and their sexual dimorphism change throughout development, the 2D:4D ratio remains stable and age-independent between ages 2 and 30.

Limits

The study relies on cross-sectional age comparisons rather than a true longitudinal design. Digit measurements in the adult cohort were self-reported rather than taken by trained experimenters. The abstract does not provide numerical values, effect sizes, or confidence intervals.

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