Bielefeld · Hearing research 2018 · controlled animal laboratory experiment · n=57

Chronotolerance for cisplatin ototoxicity in the rat.

Cited 17 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal experimental study

PubMed 30253329 · doi:10.1016/j.heares.2018.09.004 · record verified 2026-08-26

What was done

Fifty-seven Fischer 344/NHsd rats maintained on a 12-hour light/12-hour dark cycle received an intraperitoneal injection of cisplatin (12 mg/kg) at one of six time points: 2, 6, or 10 hours after light onset (inactive phase) or 2, 6, or 10 hours after light offset (active phase). Cochlear injury was assessed via auditory brainstem response threshold shifts and postmortem outer hair cell counts.

What was found

All animals showed threshold shifts at high frequencies (30 and 40 kHz). Rats treated 6 hours after light onset (inactive phase) had significantly higher mid-frequency threshold shifts and greater outer hair cell loss compared to rats treated during the dark hours. Exact numerical values and statistics were not reported in the abstract.

Why it matters

This study demonstrates that circadian timing modulates cisplatin ototoxicity in rodents, mirroring established patterns of chronotolerance for nephrotoxicity and providing a rationale for investigating chronotherapy in human cancer treatment.

Limits

The study was conducted in a nocturnal rodent model using intraperitoneal administration, which cannot be directly translated to diurnal human patients or clinical dosing protocols. The abstract provides no exact quantitative values, variance, or p-values, and it did not evaluate whether chronotolerance alters anti-tumor efficacy.

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