Mosaicism in CRISPR/Cas9-mediated genome editing.
Level 5 - mechanism / opinion, no new human data
Narrative review of mechanistic and pre-clinical animal embryo genome editing.
PubMed 30359560 · doi:10.1016/j.ydbio.2018.10.008
What was done
This is a narrative review examining the mechanisms, advantages, disadvantages, and potential reduction strategies for mosaic mutations resulting from CRISPR/Cas9-mediated genome editing in animal embryos.
What was found
The abstract reports no numerical outcomes. It qualitatively describes mosaicism as a common obstacle in founder animals generated via embryonic CRISPR/Cas9 editing, outlines underlying mechanisms, and discusses approaches to reduce mosaicism to improve the reliability of germline genotyping.
Why it matters
Addressing embryonic mosaicism is essential for reliable generation of animal models and for advancing the safety and precision of clinical genome editing applications.
Limits
The record is a non-systematic narrative overview with no empirical data, quantitative findings, sample sizes, or search criteria provided.
Cited by
- context If a genetic edit is performed on an embryo, every developing cell in that embryo will carry the edit, including the germline cells (sperm and egg).