Williams · Molecular and cellular endocrinology 2019 · In vitro controlled laboratory study · n=?

Low-dose environmental endocrine disruptors, increase aromatase activity, estradiol biosynthesis and cell proliferation in human breast cells.

Cited 78 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

In vitro laboratory study evaluating cellular mechanisms

PubMed 30817981 · doi:10.1016/j.mce.2019.02.016 · record verified 2026-08-29

What was done

Researchers exposed three human breast cell lines (MCF-7 and ZR-75-1 breast cancer cells, and HMF3A breast fibroblasts) to environmentally relevant concentrations of seven phenolic endocrine-disrupting compounds (EDCs): p,p'-DDT, methoxychlor, benzophenone-2, bisphenol A, bisphenol S, 4-phenylphenol, and n-butylparaben. Assays were conducted with and without aromatase inhibitors and estrogen receptor inhibitors to evaluate aromatase CYP19A1 mRNA expression, aromatase activity, 17β-estradiol biosynthesis, and cell proliferation.

What was found

No quantitative values or effect sizes were reported in the abstract. Qualitatively, all seven tested EDCs up-regulated aromatase mRNA, increased aromatase activity, significantly elevated aromatase-induced 17β-estradiol biosynthesis, and increased proliferation in ERα-positive breast cells.

Why it matters

This study provides mechanistic in vitro evidence that low, environmentally relevant doses of widespread phenolic compounds can enhance local estrogen synthesis and stimulate breast cancer cell growth.

Limits

The study is entirely in vitro, which does not account for systemic metabolism, pharmacokinetics, or physiological tissue interactions in vivo. The abstract omits specific numeric concentrations, effect sizes, and statistical variance.

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