Esteves · Frontiers in endocrinology 2019 · retrospective cohort prediction model development study · n=?

A Novel Predictive Model to Estimate the Number of Mature Oocytes Required for Obtaining at Least One Euploid Blastocyst for Transfer in Couples Undergoing in vitro Fertilization/Intracytoplasmic Sperm Injection: The ART Calculator.

Cited 95 times in the scientific literature.

Level 3 - non-randomized controlled study

Non-randomized cohort study developing and internally validating a clinical prediction model

PubMed 30873117 · doi:10.3389/fendo.2019.00099 · record verified 2026-08-26

What was done

Clinical and embryonic data from infertile couples undergoing IVF/ICSI with trophectoderm biopsy for preimplantation genetic testing for aneuploidy were analyzed. Negative binomial modeling and adaptive LASSO were used for variable selection, and a multivariable logistic regression model was constructed to predict the probability of obtaining at least one euploid blastocyst. The model was internally validated using the holdout method.

What was found

The final model identified female age, sperm source used for ICSI, and the number of mature (metaphase II) oocytes as significant predictors (p < 0.0001). Female age was the strongest predictor (loglikelihood: 30.9; df = 2; p < 0.0001). The predictive model achieved an area under the ROC curve of 0.716. Testicular sperm use negatively modulated the probability across age groups (p < 0.001). The abstract does not report the participant count or absolute oocyte numbers.

Why it matters

The resulting "ART calculator" provides individualized pre-treatment and post-retrieval estimates of the mature oocytes needed to achieve at least one euploid embryo for transfer.

Limits

The abstract omits sample size, patient demographics, and center details. The model underwent only internal holdout validation without external testing, and it predicts an intermediate embryological marker rather than live birth rates.

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