Inoue · Cells 2019 · in vitro controlled cell culture study · n=?

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Cited 77 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

In vitro bench research using mouse microglia

PubMed 31159306 · doi:10.3390/cells8060527 · record verified 2026-08-26

What was done

Investigators treated mouse microglia with lipopolysaccharide (LPS) to model neuroinflammation and endoplasmic reticulum (ER) stress. They evaluated the involvement of the eIF-2α-ATF4 pathway in cytokine production and tested whether oxytocin administration suppresses LPS-mediated inflammatory responses.

What was found

LPS stimulation activated the ER stress-related eIF-2α-ATF4 pathway, which promoted TNF-α, IL-6, and inflammasome-mediated IL-1β production in mouse microglia. Oxytocin treatment suppressed the production of these proinflammatory cytokines by inhibiting the activation of the eIF-2α-ATF4 pathway. The abstract reports no numerical values, concentrations, or statistical metrics.

Why it matters

This study identifies the eIF-2α-ATF4 pathway as a mechanistic mediator of microglial inflammation and demonstrates that oxytocin can suppress this pathway in vitro, suggesting a potential target for neuroinflammatory modulation.

Limits

Findings are restricted to in vitro mouse microglial cell cultures and cannot be directly translated to human neuroinflammation. The abstract provides no quantitative data, sample sizes, experimental replicates, or in vivo functional validations.

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