Zaramela · Nature microbiology 2019 · Preclinical metagenomic, biochemical, and structural study · n=?

Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates.

Cited 88 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical bench, animal, and metagenomic research with no human clinical trial data

PubMed 31548686 · doi:10.1038/s41564-019-0564-9 · record verified 2026-08-29

What was done

Researchers examined the impact of a diet rich in N-glycolylneuraminic acid (Neu5Gc) on the gut microbiota using mouse and human shotgun metagenomic sequencing. They identified bacterial sialidases, solved their structural basis for substrate preference using X-ray crystallography, and tested whether these enzymes from mouse and human gut bacteria could release Neu5Gc from red meat.

What was found

The abstract reports no numerical values. A Neu5Gc-rich diet altered gut microbial composition, with Bacteroidales and Clostridiales showing the largest response. Bacterial sialidases encoded by these microbes demonstrated substrate preference for Neu5Gc-containing glycans, mediated by key amino acids identified via crystallography. Both mouse- and human-derived sialidases successfully cleaved and released Neu5Gc from red meat.

Why it matters

Identifying gut bacterial enzymes capable of selectively cleaving dietary Neu5Gc provides a mechanistic basis for how the microbiome processes meat-derived glycans and highlights a potential strategy to reduce inflammatory risks linked to red meat intake.

Limits

The abstract provides no sample sizes, demographic details, or quantitative effect sizes. Findings are restricted to preclinical metagenomic, crystallographic, and in vitro models, leaving in vivo human health outcomes and disease risk reductions unmeasured.

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