Salehpour · Photobiomodulation, photomedicine, and laser surgery 2019 · Literature review · n=?

Penetration Profiles of Visible and Near-Infrared Lasers and Light-Emitting Diode Light Through the Head Tissues in Animal and Human Species: A Review of Literature.

Cited 154 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative literature review of preclinical animal and human tissue penetration studies

PubMed 31553265 · doi:10.1089/photob.2019.4676 · record verified 2026-08-26

What was done

The authors reviewed published literature on the penetration profiles of visible and near-infrared (NIR) laser and light-emitting diode (LED) light (630–810 nm) through head tissues in animal and human species. Light penetration data were evaluated across factors including wavelength, coherence, beam characteristics, anatomical irradiation site, tissue layers examined, species, age, and sex.

What was found

Average transcranial red/NIR (630–810 nm) penetration rates across species were: - C57BL/6 mouse (skull only): 60–70% - BALB/c mouse (skull only): 1–10% (1.75% for 810 nm laser through intact skull) - Sprague-Dawley rats (scalp plus skull): 10–40% - Oryctolagus cuniculus rabbit (skull only): 20% - Pig (scalp plus skull): 0.11% (for 808 nm laser) - Humans (scalp plus skull): 0.2–10% The large variation in reported penetration was driven by differences in tissue thickness, anatomical site, wavelength, and light coherence.

Why it matters

Translatability of transcranial photobiomodulation from small animal models to humans is severely constrained by head tissue thickness, as human scalp and skull attenuate over 90% to 99.8% of applied red/NIR light.

Limits

The abstract does not state the number of included studies or systematic search methodology. Direct comparison between models is limited by heterogeneous experimental setups, differing tissue preparations (skull alone versus scalp plus skull), and varied laser/LED parameters.

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