Das · Cell metabolism 2020 · preclinical mechanistic study and high-throughput screen · n=?

Microbial Metabolite Signaling Is Required for Systemic Iron Homeostasis.

Cited 339 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical bench research and mechanistic laboratory screen without human clinical data.

PubMed 31708445 · doi:10.1016/j.cmet.2019.10.005 · record verified 2026-08-26

What was done

Conducted a high-throughput screen of microbial metabolites to identify mechanisms by which indigenous gut bacteria influence host iron transport and storage. Evaluated the molecular actions of specific candidate metabolites (1,3-diaminopropane [DAP] and reuterin) on hypoxia-inducible factor 2α (HIF-2α) activity, heterodimerization, ferritin expression, and systemic iron regulation.

What was found

The abstract reports no numerical values, effect sizes, or statistical metrics. Qualitatively, gut microbial metabolites (specifically DAP and reuterin) were found to inhibit HIF-2α heterodimerization, suppress intestinal iron absorption, increase the iron-storage protein ferritin, and reduce systemic iron overload.

Why it matters

This study reveals a direct metabolic signaling pathway between the gut microbiome and host iron regulatory machinery. Identifying DAP and reuterin as inhibitors of intestinal iron uptake outlines potential mechanistic targets for treating systemic iron overload disorders.

Limits

All findings are derived from preclinical screening and laboratory models; the abstract reports no human data or clinical validation. No quantitative metrics, concentrations, or sample sizes are provided in the abstract. Potential systemic off-target effects of inhibiting HIF-2α via these metabolites were not characterized in the text.

Cited by