Carlezon · Scientific reports 2019 · Controlled laboratory animal experiment · n=?

Maternal and early postnatal immune activation produce sex-specific effects on autism-like behaviors and neuroimmune function in mice.

Cited 149 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal research with no human clinical data.

PubMed 31729416 · doi:10.1038/s41598-019-53294-z · record verified 2026-08-26

What was done

Timed-pregnant C57BL/6J mice received polyinosinic:polycytidylic acid (Poly I:C) at gestational day 12.5 to produce maternal immune activation (MIA). A subset of offspring received lipopolysaccharide (LPS) on postnatal day 9 for postnatal immune activation (PIA), establishing prenatal, postnatal, and combined two-hit immune activation regimens. Offspring were assessed for autism spectrum disorder-like phenotypes (social interaction, repetitive behavior, ultrasonic vocalizations) and brain expression of pro-inflammatory and anti-inflammatory mRNA and protein.

What was found

The abstract provides no numerical data, effect sizes, or statistical metrics. Early immune activation produced disruptions in social behavior and increases in repetitive behavior that were larger in males than in females. Ultrasonic vocalizations were altered in both sexes. Both sexes showed increases in pro-inflammatory mRNA and protein, whereas expression of anti-inflammatory factors decreased in males but increased in females.

Why it matters

The study outlines a potential neuroimmune mechanism for male-biased vulnerability and female resilience following early-life immune challenge in a model of autism spectrum disorder.

Limits

Findings are based entirely on an animal model and cannot directly establish human etiology. The abstract omits sample sizes, quantitative measurements, effect sizes, p-values, and the specific molecular names of the assayed inflammatory markers.

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