Cintron-Colon · Current biology : CB 2019 · animal experimental and chemogenetic study · n=?

Activation of Kappa Opioid Receptor Regulates the Hypothermic Response to Calorie Restriction and Limits Body Weight Loss.

Cited 37 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal and mechanistic laboratory study without human clinical data

PubMed 31786059 · doi:10.1016/j.cub.2019.10.027 · record verified 2026-08-30

What was done

Researchers investigated the role of kappa opioid receptor (KOR) signaling in regulating core body temperature and weight loss during calorie restriction (CR) in lean and diet-induced obese (DIO) mice. They evaluated hypothalamic Leu-enkephalin levels, tested pharmacological blockade of KOR, delta, and mu opioid receptors in CR and ad libitum-fed mice, and used chemogenetics to inhibit preoptic KOR neurons or activate prodynorphin-expressing neurons in the arcuate and parabrachial nuclei.

What was found

The abstract reports no numerical values, effect sizes, or exact temperature changes. Calorie restriction increased hypothalamic Leu-enkephalin levels. Pharmacological inhibition of KOR, but not delta or mu opioid receptors, fully blocked CR-induced hypothermia and increased weight loss during CR in both lean and DIO mice without changing caloric intake. KOR inhibition did not alter core body temperature in mice fed ad libitum. Chemogenetic inhibition of preoptic KOR neurons reduced CR-induced hypothermia, while activation of prodynorphin neurons in the arcuate or parabrachial nucleus decreased core body temperature.

Why it matters

This study identifies central KOR signaling as a critical regulator of adaptive hypothermia and energy conservation during food scarcity. Understanding this pathway may inform interventions aimed at overcoming metabolic adaptation during weight loss.

Limits

The study was conducted exclusively in rodent models, so direct applicability to human energy homeostasis is unknown. The abstract does not provide sample sizes, quantitative measurements of body temperature or weight change, or variance estimates.

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