Guarnotta · The Journal of steroid biochemistry and molecular biology 2020 · case-control study · n=101

Clinical and hormonal characteristics in heterozygote carriers of congenital adrenal hyperplasia.

Cited 27 times in the scientific literature.

Level 4 - case-series / case-control

Case-control observational study comparing heterozygous carriers, affected patients, and healthy controls

PubMed 31805392 · doi:10.1016/j.jsbmb.2019.105554 · record verified 2026-08-31

What was done

Clinical, biochemical, and genetic characteristics were evaluated in a cohort of 57 Sicilian females with CYP21A2 mutations (24 with non-classical congenital adrenal hyperplasia [NC-CAH] bearing biallelic variants and 33 heterozygous CAH carriers bearing monoallelic variants) alongside 44 age-matched healthy female controls.

What was found

CYP21A2 heterozygous carriers presented with clinical features such as hirsutism, oligomenorrhoea, overweight, and a polycystic ovary (PCO)-like phenotype, especially manifesting in adolescence. Hormonal levels of 17-hydroxyprogesterone (17OHP) and cortisol in carriers were significantly different from NC-CAH patients. Oligomenorrhea and the 17OHP/cortisol ratio were identified as independent markers associated with carrier status. The abstract reports no exact numerical hormone levels, odds ratios, or diagnostic cut-offs.

Why it matters

This study highlights that monoallelic CYP21A2 mutations are not purely silent and can present with late-onset hyperandrogenic or PCOS-like features, proposing the 17OHP/cortisol ratio as a potential biochemical screening tool.

Limits

The abstract reports no numerical values, confidence intervals, or defined cut-offs for the 17OHP/cortisol ratio. The sample size is modest (33 carriers, 101 total subjects) and derived exclusively from a single regional cohort (Sicily), limiting broader generalizability.

Cited by