Souza · Circulation research 2020 · Randomized double-blind placebo-controlled crossover trial · n=?

Enriched Marine Oil Supplements Increase Peripheral Blood Specialized Pro-Resolving Mediators Concentrations and Reprogram Host Immune Responses: A Randomized Double-Blind Placebo-Controlled Study.

Cited 157 times in the scientific literature.

Level 2 - randomized trial

Double-blind, placebo-controlled crossover randomized trial in humans

PubMed 31829100 · doi:10.1161/CIRCRESAHA.119.315506 · record verified 2026-08-29

What was done

Healthy volunteers participated in a double-blind, placebo-controlled crossover study evaluating three different doses of an enriched marine oil supplement against placebo. Blood samples were collected at baseline, 2, 4, 6, and 24 hours after administration. Investigators performed lipid mediator profiling to track peripheral blood specialized pro-resolving mediator (SPM) concentrations, evaluated neutrophil and monocyte phagocytosis of bacteria, quantified leukocyte and platelet adhesion molecule expression, and conducted transcriptomic profiling of peripheral blood cells at 24 hours.

What was found

The abstract provides no exact numerical values, effect sizes, or confidence intervals. It reports that marine oil supplementation resulted in a time- and dose-dependent increase in peripheral blood SPM concentrations, a dose-dependent increase in bacterial phagocytosis by neutrophils and monocytes, a decrease in diurnal leukocyte and platelet activation as indicated by reduced adhesion molecule expression, and transcriptomic shifts in immune and metabolic genes at 24 hours relative to placebo.

Why it matters

The study provides human trial evidence that oral marine oil supplementation directly elevates circulating specialized pro-resolving mediators and acutely alters leukocyte functional phenotypes. This establishes a direct mechanistic pathway connecting omega-3 fatty acid intake to systemic pro-resolving and anti-inflammatory immune modulation.

Limits

The abstract does not disclose the sample size, participant demographics, exact doses administered, or quantitative effect sizes and statistical metrics. The evaluation was limited to healthy volunteers over a single 24-hour observation window, precluding conclusions about sustained long-term administration, chronic inflammation, or clinical disease outcomes.

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