Bertoldo · Cell reports 2020 · Preclinical animal and laboratory study · n=?

NAD + Repletion Rescues Female Fertility during Reproductive Aging.

Cited 331 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and bench research with no human clinical data.

PubMed 32049001 · doi:10.1016/j.celrep.2020.01.058 · record verified 2026-08-26

What was done

Researchers investigated whether reproductive aging and declining oocyte quality in female mammals correlate with reduced nicotinamide adenine dinucleotide (NAD+) levels. They administered the NAD+ precursor nicotinamide mononucleotide (NMN) to aged animals to evaluate changes in oocyte quality and fertility, examined the effects of transgenic SIRT2 overexpression and deletion, and assessed embryonic developmental milestones following supplementation.

What was found

The abstract reports no numerical data, effect sizes, or sample sizes. Treatment with NMN rejuvenated oocyte quality and restored fertility in aged animals, while reversing maternal-age-related developmental delays in developing embryos. Transgenic overexpression of SIRT2 recapitulated these improvements in oocyte quality, whereas SIRT2 deletion did not impair oocyte quality.

Why it matters

This study links maternal age-related oocyte decline to NAD+ depletion in animal models, suggesting that metabolic precursor supplementation or SIRT2 pathways might represent viable targets to support female reproductive function.

Limits

The findings are based strictly on preclinical animal and laboratory experiments without human data. The abstract provides no specific numbers, sample sizes, animal species identification, treatment dosages, or statistical effect sizes.

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