Yang · Free radical biology & medicine 2020 · Controlled animal experiment · n=?

Increasing ovarian NAD + levels improve mitochondrial functions and reverse ovarian aging.

Cited 127 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal (mouse) interventional study

PubMed 32492457 · doi:10.1016/j.freeradbiomed.2020.05.003 · record verified 2026-08-26

What was done

Researchers measured ovarian nicotinamide adenine dinucleotide (NAD+) levels across age in female mice and evaluated the effects of supplementation with the NAD+ precursor nicotinamide riboside (NR) in vivo and in vitro. They assessed ovarian NAD+ concentration, follicle counts, ovulatory capacity, live birth rates, oocyte reactive oxygen species levels, spindle abnormalities, mitochondrial membrane potential, mitochondrial clustering, and mitochondrial energy metabolism.

What was found

The abstract reports no exact numerical values or confidence intervals. It states that ovarian NAD+ declined with age, and NR supplementation restored ovarian NAD+ content in aging mice. NR treatment resulted in increased follicle counts, enhanced ovulatory potential, higher live birth rates, reduced reactive oxygen species, decreased spindle anomalies, increased mitochondrial membrane potential, decreased mitochondrial clustering, and improved mitochondrial energy metabolism.

Why it matters

This study provides preclinical mechanistic evidence that age-related decline in ovarian NAD+ impairs oocyte quality and mitochondrial function, suggesting NAD+ precursor supplementation as a candidate strategy for female age-related infertility.

Limits

The study was conducted entirely in mice, so findings cannot be directly extrapolated to human reproductive outcomes. The abstract does not report sample sizes, dosage, duration of NR administration, statistical effect sizes, or quantitative outcome metrics.

Cited by