Griffiths · Biochemical Society transactions 2020 · narrative review · n=?

Nicotinamide adenine dinucleotide (NAD+): essential redox metabolite, co-substrate and an anti-cancer and anti-ageing therapeutic target.

Cited 56 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review of mechanistic and preclinical research without systematic methodology or new human data.

PubMed 32573651 · doi:10.1042/BST20190033 · record verified 2026-08-26

What was done

This narrative review summarizes the biological roles of nicotinamide adenine dinucleotide (NAD+) and NADH in redox metabolism and as catalytic co-substrates for enzymes including sirtuins and PARPs. It reviews NAD+ homeostasis, the age-related decline of NAD+ in tissues, dietary supplementation with NAD+ precursors, cancer dependency on NAD+, therapeutic targeting of NAMPT in the tumor microenvironment, and techniques for measuring subcellular NAD+ dynamics.

What was found

The abstract reports no quantitative values or statistical effect sizes. It qualitatively describes that NAD+ concentrations decrease with age in the human brain and peripheral organs, correlating with neurodegeneration, while many malignancies show heightened dependency on NAD+ metabolism. The author notes that clinical attempts to therapeutically exploit NAD+ metabolism in cancer have achieved limited success so far.

Why it matters

It highlights the contrasting therapeutic opportunities in NAD+ biology: elevating NAD+ pools to mitigate age-related diseases versus disrupting NAD+ synthesis pathways to inhibit tumor metabolism.

Limits

As a narrative review, it presents mechanistic syntheses rather than systematic review metrics or new empirical clinical trials. No sample sizes, effect estimates, or formal risk-of-bias evaluations are reported in the abstract.

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