Prasad · The Journal of clinical investigation 1988 · Before-after experimental depletion and cohort supplementation study · n=14

Serum thymulin in human zinc deficiency.

Cited 341 times in the scientific literature.

Level 4 - case-series / case-control

Small non-randomized before-after experimental and observational series

PubMed 3262625 · doi:10.1172/JCI113717 · record verified 2026-08-26

What was done

Researchers measured serum thymulin activity and immune cell parameters before and after zinc supplementation across three groups of adults with mild zinc deficiency diagnosed via leukocyte and platelet zinc levels (despite normal plasma zinc): 2 volunteers undergoing experimental dietary zinc depletion, 6 subjects with sickle cell anemia, and 6 non-sickle cell anemia subjects.

What was found

The abstract reports directional changes without numeric values or statistical confidence intervals. Serum thymulin activity decreased during mild zinc deficiency and was restored by in vivo and in vitro zinc supplementation. In the experimental dietary depletion model, mild deficiency led to an increase in T101- sIg- cells, a decrease in the T4+/T8+ ratio, and decreased IL-2 activity, all of which reversed with zinc repletion. Similar reversible alterations in lymphocyte subpopulations occurred in the sickle cell group.

Why it matters

The study suggests serum thymulin activity is a sensitive functional marker of mild cellular zinc deficiency even when routine plasma zinc concentrations appear normal, providing a biological mechanism for zinc-dependent T-cell dysfunction.

Limits

Sample size is extremely small (total n = 14 across three distinct groups, with only 2 dietary depletion subjects). The study lacked a randomized control group or blinding, and the abstract provides no numerical data, baseline values, or p-values.

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