Naghshi · BMJ (Clinical research ed.) 2020 · Systematic review and dose-response meta-analysis of prospective cohort studies · n=32 studies (715,128 participants)

Dietary intake of total, animal, and plant proteins and risk of all cause, cardiovascular, and cancer mortality: systematic review and dose-response meta-analysis of prospective cohort studies.

Cited 369 times in the scientific literature.

Level 3 - non-randomized controlled study

Systematic review and meta-analysis of prospective cohort studies

PubMed 32699048 · doi:10.1136/bmj.m2412 · record verified 2026-08-28

What was done

Systematic review and dose-response meta-analysis of prospective cohort studies searched across PubMed, Scopus, and ISI Web of Science up to December 2019. Eligible studies evaluated the association between dietary intake of total, animal, and plant protein and all-cause, cardiovascular disease (CVD), and cancer mortality in adults aged 18 or older. Random-effects models were used to calculate pooled effect sizes comparing highest versus lowest intake categories, and linear and non-linear dose-response analyses were performed.

What was found

Across 32 prospective cohort studies (31 in meta-analysis) with 715,128 participants and 113,039 deaths (16,429 from CVD, 22,303 from cancer) followed for 3.5 to 32 years: - Total protein intake was associated with lower all-cause mortality (pooled effect size 0.94, 95% CI 0.89 to 0.99, I² = 58.4%, P < 0.001), but had no significant association with CVD or cancer mortality. - Plant protein intake was associated with lower all-cause mortality (pooled effect size 0.92, 95% CI 0.87 to 0.97, I² = 57.5%, P = 0.003) and lower CVD mortality (pooled HR 0.88, 95% CI 0.80 to 0.96, I² = 63.7%, P = 0.001), but not cancer mortality. - Dose-response analysis indicated that an additional 3% energy per day from plant protein was associated with a 5% lower risk of all-cause mortality (P = 0.05 for non-linearity). - Total and animal protein intakes were not significantly associated with CVD or cancer mortality.

Why it matters

This synthesis of prospective cohort evidence demonstrates that higher plant protein intake is linked to reduced all-cause and cardiovascular mortality, supporting dietary recommendations that emphasize plant-based protein sources.

Limits

The findings are derived entirely from observational prospective cohort studies, leaving potential for residual confounding by lifestyle factors. Moderate between-study heterogeneity was observed (I² ranged from 57.5% to 63.7%). Specific food sources within broad protein categories, measurement error from dietary assessments, and subgroup differences were not detailed in the abstract.

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