Kankaanpää · Medicine and science in sports and exercise 2021 · cross-sectional twin study · n=520 twin pairs

Leisure-Time and Occupational Physical Activity Associates Differently with Epigenetic Aging.

Cited 75 times in the scientific literature.

Level 3 - non-randomized controlled study

Cross-sectional twin study with quantitative genetic modeling

PubMed 32868581 · doi:10.1249/MSS.0000000000002498 · record verified 2026-08-30

What was done

Researchers evaluated the cross-sectional association between physical activity domains and blood DNA methylation (DNAm) age acceleration across three clocks: DNAm age, PhenoAge, and GrimAge. Participants were 285 young adult twin pairs (aged 21–25 yr) and 235 older twin pairs (aged 55–74 yr), including 16 pairs discordant for long-term leisure-time physical activity. Physical activity was assessed with sport, leisure-time, and work indices using the Baecke Questionnaire. Genetic and environmental variance components were estimated with quantitative genetic modeling.

What was found

Epigenetic age acceleration was approximately 60% heritable in both cohorts. In sex-adjusted models, higher sport index was associated with slower GrimAge acceleration, whereas occupational physical activity was associated with faster GrimAge acceleration. Genetic and nonshared environmental factors shared with sport index explained 1.5%–2.7% and 1.9%–3.5% of variance in GrimAge acceleration, respectively; for occupational activity, corresponding proportions were 0.4%–1.8% and 0.7%–1.8%. After adjusting for smoking status, all explained variance proportions fell below 0.5%.

Why it matters

This study provides biological evidence supporting the physical activity paradox—that occupational physical activity does not confer the same anti-aging profile as leisure exercise—while demonstrating that lifestyle confounders like smoking account for most of the apparent association.

Limits

The study is cross-sectional, preventing causal conclusions. Physical activity was self-reported via questionnaires rather than measured objectively. The discordant twin subsample was small (16 pairs), and the vast majority of observed variance explained by physical activity disappeared after controlling for smoking status.

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