Moraes · Experimental gerontology 2020 · Controlled laboratory animal study · n=?

Oral benfotiamine reverts cognitive deficit and increase thiamine diphosphate levels in the brain of a rat model of neurodegeneration.

Cited 23 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model (rodent study)

PubMed 32987117 · doi:10.1016/j.exger.2020.111097 · record verified 2026-08-29

What was done

Researchers administered oral benfotiamine (150 mg/kg daily for 30 days) to a rat model of sporadic Alzheimer's disease induced by intracerebroventricular injection of streptozotocin. They evaluated brain thiamine absorption (thiamine diphosphate concentrations in the hippocampus and entorhinal cortex), mitochondrial enzyme activity, insulin signaling pathways, tau-kinase activation (GSK3α/β and ERK1/2), NMDA receptor GluN2B subunits, inflammation, and cognitive performance.

What was found

The abstract reports directional outcomes without numerical data, effect sizes, or p-values. Benfotiamine supplementation increased thiamine diphosphate in the hippocampus and entorhinal cortex, improved mitochondrial enzyme activity and insulin signaling, inactivated the tau-kinases GSK3α/β and ERK1/2, increased GluN2B subunit expression, reduced inflammation, and reversed cognitive deficits.

Why it matters

This study provides preclinical evidence that a highly bioavailable thiamine derivative can penetrate relevant brain regions and counteract several metabolic, kinase-mediated, and cognitive deficits in a neurodegenerative model.

Limits

The study was conducted entirely in a chemically induced rodent model (intracerebroventricular streptozotocin), which does not fully capture human Alzheimer's disease biology. The abstract does not report the total sample size (n), precise quantitative measurements, variance, or statistical significance metrics. The intervention was evaluated over a single 30-day period with one dose level.

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