Jolliffe · The lancet. Diabetes & endocrinology 2021 · systematic review and meta-analysis of RCTs · n=46 studies (75,541 participants)

Vitamin D supplementation to prevent acute respiratory infections: a systematic review and meta-analysis of aggregate data from randomised controlled trials.

Cited 507 times in the scientific literature.

Level 1 - systematic review of randomized trials

Systematic review and meta-analysis of randomized controlled trials

PubMed 33798465 · doi:10.1016/S2213-8587(21)00051-6 · record verified 2026-08-29

What was done

Authors conducted an updated systematic review and random-effects meta-analysis of double-blind randomized controlled trials (searched through May 1, 2020) evaluating vitamin D supplementation (vitamin D3, D2, or 25-hydroxyvitamin D) versus placebo or low-dose control. The primary outcome was the proportion of participants experiencing one or more acute respiratory infections (ARIs). Subgroup analyses evaluated baseline 25(OH)D concentration, dosing regimen and frequency, study duration, age, and underlying airway disease. Serious adverse events and risk of bias were also evaluated.

What was found

Out of 46 eligible RCTs with 75,541 participants, primary outcome data across 37 studies showed vitamin D significantly lowered the odds of having one or more ARIs compared to placebo: 61.3% (14,332/23,364) versus 62.3% (14,217/22,802), corresponding to an OR of 0.92 (95% CI 0.86-0.99; I2=35.6%, p=0.018). Subgroup analyses found significant protective effects for daily dosing (OR 0.78, 95% CI 0.65-0.94; 19 studies), daily dose equivalents of 400-1000 IU (OR 0.70, 95% CI 0.55-0.89; 10 studies), trial durations of 12 months or less (OR 0.82, 95% CI 0.72-0.93; 29 studies), and ages 1.00-15.99 years (OR 0.71, 95% CI 0.57-0.90; 15 studies). No significant subgroup effects were found based on baseline 25(OH)D levels, and formal interaction tests between subgroups were not statistically significant. Serious adverse events did not differ between groups (OR 0.97, 95% CI 0.86-1.07; 36 studies).

Why it matters

This updated meta-analysis confirms that vitamin D supplementation provides a safe, modest reduction in acute respiratory infection risk across broad populations, with the clearest benefits observed with daily moderate dosing.

Limits

The overall absolute risk reduction was very small (approximately 1 percentage point). Significant heterogeneity existed among included trials. The analysis relied on aggregate study-level data rather than individual participant data, and pathogen-specific outcomes (including SARS-CoV-2) were not evaluated.

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